Coding

Part:BBa_K4161506

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-26)


332aa, ipaD protein

This sequence expresses the ipaD protein of the bacteria Shigella. IpaD is the needle tip protein residing at the tip of the Shigella T3SA. It controls the secretion of subsequent type III secretion system proteins in Shigella.


Usage and Biology

Biology

Type III secretion systems comprise common virulence factors in gram-negative bacteria that plays an important role in transporting bacterial effector proteins into the host cytoplasm. In Shigella, this translocation process requires three translocator proteins including the IpaD protein.

Usage

In our project, we express is protein to test the binding affinity between the antibody 20ipaD and the antigen ipaD.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 708


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Categories
Parameters
None